Microbial and Synthetic Biology
Capture what matters
Analysing bacteria and other microorganisms poses many challenges for classic droplet microfluidic
techniques. The first step of harsh cell lysis is often not only incompatible with droplet
stability, but also interferes with downstream molecular biology reactions. To overcome this, we
have developed the semi-permeable droplet technology, which
allows to change reaction composition,
while maintaining compartmentalization of single bacterial cells.
Our semi-permeable droplet technology is the solution for
multi-step molecular analysis.
Targeted Gene Analysis
Measure the abundance of target genes by measuring fluorescence
Speed up the design-build-test cycle and explore the
possibilities of multi-step workflows.
Cultivate diverse microorganisms in their natural environment
while maintaining compartmentalization.
Our easy-to-use and versatile semi-permeable droplet system
is based on hydrogel shell formation. It
works like a semi-permeable membrane and enables a steady
exchange of small molecular weight
components, while retaining large molecules inside the droplet. This makes multi-step reactions,
extended culture in native environments and most importantly, labelling and selection of species of
We anticipate that the relatively simple generation of semi-permeable
capsules presented here will facilitate the development of new single-cell assays in biology.
Adding and washing reagents from our semi-permeable droplets do not require any special equipment. This
process is as easy as spinning droplets down with a table-top centrifuge and changing the surrounding buffer
The Onyx is a fully integrated microfluidic platform, that makes the
adopting the semi-permeable droplet
technology easy. The instrument is also flexible and open making it future proof and compatible novel