
multi-step molecular analysis in a droplet format.
Analysing bacteria and other microorganisms poses many challenges for classic droplet microfluidic techniques. The first step of harsh cell lysis not only interferes with droplet stability but is also often incompatible with downstream molecular biology reactions.
To overcome this, we have developed the semi-permeable capsule technology, which allows performing multi-step workflows while maintaining compartmentalisation of single cells.
Semi-Permeable Capsules (SPCs)
Our easy-to-use and versatile Semi-Permeable Capsule technology is based on particles with a hydrogel shell. The shell works like a size-selective membrane and enables a steady exchange of small molecular weight components all while retaining large molecules and cells inside the droplets and maintaining sample compartmentalisation. This makes multi-step reactions, compartmentalised liquid culture and, most importantly, labelling and selecting molecular and cellular species of interest possible.
“We anticipate that the relatively simple generation of semi-permeable capsules presented here will facilitate the development of new single-cell assays in biology.”
G. Leonaviciene et. al. Multi-step processing of single cells using semi-permeable capsules.



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